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Webb5 nov. 2024 · The real-time PCR protocol recognized all C. siamense isolates collected from three provinces in China, whereas no amplification was observed with the rubber … Webb1 jan. 2024 · The amplified fragments were fused together by overlap PCR and inserted into pNV-Pa2. Pa2 was a urea-inducible promoter, and its expression relied on native transcriptional regulators in the genome of R. ruber. pNVTc was a derivative of pNV18.1 generated by replacing the Km R gene with the Tc R gene. rock band tongue
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