Phis2自激活

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August undefined, 2024

Webb19 nov. 2012 · 是选取atg上游2000的片段，然后整个连到phis2载体上吗？我之前做了直接取ATG上游1.5k构建到phis2上，感觉不合适。最好还是找到自己转录因子的结合motif … http://www.zoonbio.com/molecular/yeast-one-hybrid.html

MdGSTF6, activated by MdMYB1, plays an essential role in

Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were used as negative controls. W boxes have been described as positive cis-acting elements upregulating transcription after binding with WRKY transcription factor. WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … hillman minx spares for sale

酵母单/双杂交（点对点）-西安淳风生物科技有限公司

WebbpHIS2-p53+pGADT7-53m（阳性对照） pHIS2+pGADT7-53m（阴性对照）服务特色：经验足周期短性价比高通量高. 客户提供. 构建酵母文库材料. 细胞样本：细胞数量＞ 1*10^7 动物样本：＞lg . 植物样本：＞ 2g 总RNA: ＞200μg. 酵母单杂筛库材料. 启动子序列含有诱饵 … Webb11 juli 2014 · 常用载体及通用测序引物,测序通用引物,华大测序通用引物,载体通用引物,petduet 1测序引物,通用引物,测序引物,pet28a测序引物,m13通用引物,细菌通用引物,病毒通用引物. VectorPrimer (F)Primer (R)抗性载体大小 (bp)EZ-TM13-47RV-MAmp+2984LZRS-RfALZRS-FLZRS-R11100p3xFLAGMV14CMV30 (834bp)CMV24 ... http://www.youbio.cn/product/vt1637 hillman minx pictures

Phis2自激活

MdGSTF6, activated by MdMYB1, plays an essential role in

WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: yeast series, yeast one hybrid vector Plasmid size: 7207bp … http://www.genecreate.cn/preferential_news/2444.html

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Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a multiple ... Webb仅使用命令激活Windows11，较安全，也不需要安装激活工具, 视频播放量 112905、弹幕量 155、点赞数 2564、投硬币枚数 2649、收藏人数 3667、转发人数 567, 视频作者爱玩的安哥, 作者简介每天学习一分钟，相关视频：Win11系统永久激活密钥，2分钟激活Win10/11 全版本激活数字权利 KMS这里都有 Github开源激活 ...

Webb上海懋康生物科技有限公司是一家涉足于生命科学和生物技术领域研究的试剂、仪器和实验室消耗品与实验服务工作，主要从事细胞生物学、植物学、分子生物学、免疫学、生物化学、蛋白组学。生物制药与诊断试剂研发生产等领域。本公司秉承“以人为本，以诚为信、合同守信”的经营理念。 http://baiyk.com.cn/ProDetail.aspx?ProID=56

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb服务时间：24小时在线客服 . 客服热线：400-000-7085

WebbThe T7 promoter in pGADT7-Rec2 can be used for in vitro transcription and translation of the HA-tagged fusion protein. It also provides a binding site for sequencing with the T7 Promoter Sequencing Primer. pGADT7-Rec2 is a shuttle vector; it can be maintained in both yeast and bacteria. It contains an autonomous replication sequence (ARS4) and ...

Webb14 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。材料： 1．启动子克隆：pHIS2-A。根据实验需求进行扩增并酶切构建载体，两端的EcoRI和. SacI酶切位点，序列如下： hillman minx cdWebb26 maj 2024 · pHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication sequence (ARS4) and TRP1 nutritional marker for replication … hillman minx series 4Webb27 mars 2024 · 酵母单杂交.ppt,酵母单杂交系统吉林大学植物科学学院 2010级硕士酵母单杂交系统原理质粒和菌株质粒：诱饵质粒pHIS2，文库质粒为pGATD7-rec2 诱饵质粒上连接有目标靶序列，最小启动子和报告基因文库质粒则和反转录得到的真核生物cDNA连接，可以表达AD杂合蛋白酵母菌株：Y187 GAL4基因是缺失型的 ... hillman motors winter havenWebb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). hillman mn cabins for rentWebb8 juli 2024 · pHis2 编号载体名称北京华越洋生物VECT2110 pHis2 pHis2 载体基本信息出品公司: Clontech 载体名称: pHis2 质粒类型: 酵母单杂交载体高拷贝/低拷贝: -- 启动子: -- 克隆方法: 多克隆位点，限制性内切酶载体大小: 7207bp 5' 测序引物及序列: -- 3' 测序引物及序列: -- 载体标签 ... hillman morning show liveWebb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were … smart fit jose higinoWebb24 apr. 2016 · Figure 酵母单杂和双杂筛选的一般步骤，更加详细单杂和双杂筛选流程图，请参考Figures BDMatchmaker TM 单杂和双杂文库筛选与构建. 如这个图表所示，As diagramshows,重组介导克隆使文库构建和筛选更快捷高效，尽管这里没有显示，双杂文库能通过酵母融合筛选（细节 ... hillman mn county