WebNov 22, 2024 · The developed method is suitable for the conjugate synthesis of oligonucleotides of different types (ribo-, deoxyribo-, 2′-O-methylribo-, and others). ... (1/9/90). Dichloroacetic acid (3%) in CH 2 Cl 2 was used as a detritylating reagent. Polymer-bound DMTr-off oligonucleotides were used for solid-phase conjugation. ... WebDichloroacetic acid 99+% 31249-30 vwr Dichloroacetic acid ≥99% 129280-1KG vwr Dichloroacetic acid ≥98.0% (by GC, titration analysis) D0308-25G vwr ... Acetonitrile, anhydrous ≥99.9% for DNA synthesis BDHVBDH85501.201 vwr Acetonitrile, low water content ≥99.5% ACS, VWR Chemicals BD ...
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Web80% acetic acid; Procedure: Dissolve the dried, deprotected oligo in 1 ml 80% acetic acid. Mix by vortexing. Incubate for 1 hour at room temperature. Dry the detritylated oligo using vacuum centrifugation. A reverse-phase desalting procedure may then be used to remove the free DMT from the oligo solution. Reference WebCleavage Reagent for DNA Synthesis 20% DEA in MeCN, Novabiochem®; Synonyms: 20% DEA in MeCN (v/v), Novabiochem®; find Sigma-Aldrich-857010 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. ... Deblocking Reagent 3% Dichloroacetic Acid in Toluene. flight u28118
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WebAcid (DNA) Intercalators: Principles, Design, Synthesis, Applications and Trends Brenno A. D. Neto 1,* and Alexandre A. M. Lapis 2 1 Laboratory of Medicinal and Technological … WebTraditionally dilute solutions of trichloroacetic acid (TCA, pK a 0.8) or dichloroacetic acid (DCA, p Ka 1.5) in dichloromethane (DCM) were used as detritylation reagent for ON synthesis. 125b, 132 Later, it was recognized that a high concentration of a weak acid (15% DCA in DCM) is more effective at driving detritylation toward completion than ... WebMar 29, 2024 · The synthesis cycle consists of four major steps (Shown in Figure 3), and culminates in the addition of a single residue to the growing oligo strand: The first step … great emperor hotel