Cst atf4 antibody
WebSep 5, 2024 · ATF4 is induced in CTLs exposed to tumor-derived factors and antigen stimulation. We sought to determine the expression of ATF4 in CD8 + T cells upon their activation or exposure to the stress stimuli and tumor-derived factors. Treatment of mouse splenic CD8 + T cells with thapsigargin, an inducer of the endoplasmic reticulum stress … WebPurified anti-ATF4 Antibody. Total cell lysates (15 µg protein) from non-treated and Tunicamycin (Tm) treated (2 µg/mL, six hours) HeLa and 293T were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-ATF4 (clone W16016A) antibody or competitor's antibody used at the ...
Cst atf4 antibody
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WebMonoclonal Antibody for studying ATF-4. Cited in 3 publications. Validated for Western Blotting, Immunofluorescence (Immunocytochemistry). Highly specific and rigorously … WebMonoclonal Antibody for studying ATF-4. Cited in 661 publications. Validated for WB, IP, IF, ChIP, ChIP, C&R. Highly specific and rigorously …
WebMar 3, 2024 · Effective treatments for patients suffering from heat hypersensitivity are lacking, mostly due to our limited understanding of the pathogenic mechanisms underlying this disorder. In the nervous system, activating transcription factor 4 (ATF4) is involved in the regulation of synaptic plasticity and … WebHowever, CS-11815 detects a band with a mass of ~49 kDa, while the expected molecular weight of ATF4 is ~38 kDa. In contrast, SC-200 is a very dirty polyclonal antibody, but it seems to detect ...
WebFeb 17, 2024 · Autophagy is a cellular process through which cells engulf and degrade damaged cytoplasmic components ().Recent studies have implicated the involvement of autophagy in the regulation of obesity and energy expenditure (15–21).For example, autophagy is reduced in the hypothalamus of aged mice or mice fed an HFD … WebJun 14, 2013 · The transcription factor activating transcription factor 4 (ATF4) belongs to the cAMP-responsive element–binding protein family, characterized by the presence of a leucine zipper dimerization domain and a basic amino acid–rich DNA binding domain ().Previous studies have shown that knocking out ATF4 gene expression in mice results …
WebNov 1, 2024 · Membranes were then incubated at 4 °C overnight with ATF4 antibody (BioLegend) at 1:1000. After washing with 1x TBST, anti-rat HRP secondary antibody (CST) was used at 1:2000. A list of commercially available antibodies tested in livers and cell lines is included in Supplementary Table 1. 2.6. Gene expression analysis.
WebATF4 Rabbit pAb (A0201) Western blot analysis of extracts of HeLa cells, using ATF4 antibody (A0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti … great light christmas fightWebJun 24, 2024 · PERK IPs were performed with 1 mg of lysate incubated with 2 µg of primary antibody (Proteintech, #20582-1AP) or the corresponding IgG (Cell Signaling Technology, #2729) overnight at 4 °C with ... great light fight abcWebOct 20, 2024 · ChIP assays with NFATc1 or ATF4 antibody demonstrated that isoproterenol increased NFATc1 and ATF4 binding to their cognate binding elements in RANKL ... MA, USA). CREB1 and phospho-CREB1 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA), and phospho-ATF4 antibody was … great light dinner recipesWebThe antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these … great light fight 2020WebActivating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor … great light fight hostsWebOverview Product name Anti-ATF-4 antibody See all ATF-4 primary antibodies Description Rabbit polyclonal to ATF-4 Host species Rabbit … great light fight meadowWebDec 25, 2024 · The membrane was blocked and then incubated overnight at 4°C with primary antibody as described previously ... antibody to phospho-T389 p70S6K1 was obtained from Cell Signaling Technology (catalog number: 9205); antibodies to REDD1 (catalog number: 10638-1-AP), Sestrin1 (catalog number: 21668-1-AP), and Sestrin2 … great light fight the christmas tree